fluorogenic dpp4 assay kit  (BPS Bioscience)

Journal: Bioorganic Chemistry

Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

doi: 10.1016/j.bioorg.2022.106092

Figure Lengend Snippet: Schematic representation for DPP-4/Substrate complex.

Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

Techniques:

Journal: Bioorganic Chemistry

Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

doi: 10.1016/j.bioorg.2022.106092

Figure Lengend Snippet: Naturally occurring phenolic compounds having DPP-4 inhibition potent activity.

Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

Techniques: Inhibition, Activity Assay

Journal: Bioorganic Chemistry

Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

doi: 10.1016/j.bioorg.2022.106092

Figure Lengend Snippet: In vivo DPP-4 activity as % of control in type2 diabetic rats, Values are mean ± SD (n = 3). ***significant (P < 0.001) compared to control diabetic group.

Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

Techniques: In Vivo, Activity Assay

Journal: Bioorganic Chemistry

Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

doi: 10.1016/j.bioorg.2022.106092

Figure Lengend Snippet: 2D binding modes of compounds (4b, 4c, 6a, 6b, 7d, 8b) and Linagliptin with DPP-4 active site.

Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Bioorganic Chemistry

Article Title: Novel hit of DPP-4Is as promising antihyperglycemic agents with dual antioxidant/anti-inflammatory effects for type 2 diabetes with/without COVID-19

doi: 10.1016/j.bioorg.2022.106092

Figure Lengend Snippet: 3D binding mode of compound 6a with DPP-4 active site.

Article Snippet: The active compounds in SLM were screened for their DPP-4 inhibition activities as previously described , using Fluorogenic DPP-4 Assay Kit (Bps Bioscience Cornerstone Court W, Ste B San Diego, CA 92121) following the manufacturer’s instructions.

Techniques: Binding Assay

Journal: Obesity Surgery

Article Title: Decrease of dipeptidyl peptidase 4 activity is associated with weight loss after bariatric surgery

doi: 10.1007/s11695-020-05200-0

Figure Lengend Snippet: Boxplot for the comparison between pre- and post-surgical values of serum DPP4 activity. * p≤0.005; °outlier

Article Snippet: Serum DPP4 activity was assayed by a commercially available kit measuring the fluorescence of the cleaved fluorogenic DPP4 substrate H-Gly-Pro-AMC using a microplate reader (Enzo Life Sciences, Inc., Lausen, Switzerland).

Techniques: Activity Assay